Supplementary Information for

Leng Han and Zhongming Zhao

"CpG islands or CpG clusters: how to identify functional GC-rich regions in a genome?"


Introduction

CpG islands (CGIs), clusters of CpG dinucleotides in GC-rich regions, are often located in the 5' end of genes and considered gene markers in vertebrate genomes. Methylation of promoter-associated CGIs plays an important role in the regulation of gene expression and carcinogenesis. This study performed systematic comparison between two major algorithms (Takai and Jones 2002 and CpGcluster 2006). The comparisons revealed that Takai and Jones' algorithm is overall more appropriate for identifying promoter-associated islands of CpGs in vertebrate genomes. The detailed CpG island and CpGcluster data and gene annotations are available.

 


CpG islands (Takai and Jones, 2002)

The program could be downloaded from http://cpgislands.usc.edu/ .

 

CGIs identified by Takai and Jones' algorithm in the human and mouse genomes:

CGIs in the human genome (NCBI build 36)

CGIs in the mouse genome (NCBI build 37)

 

Each column displays:

 

-Species and chromosome

-CGI ID

-Start position

-End position

-GC content (%)

-ObsCpG/ExpCpG

-Length


CpG clusters (Hackenberg et al. 2006)

The program could be downloaded from http://bioinfo2.ugr.es/CpGcluster.

CpG clusters identified by Hackenberg et al.'s algorithm in the human and mouse genomes:

CpG clusters in the human genome (NCBI build 36)

CpG clusters in the mouse genome (NCBI build 37)

Each column displays:

-CpG cluster ID

-Start position

-End position

-Length

-GC content (%)

- ObsCpG/ExpCpG

-Number of CpGs

-Mean distance between CpGs

-P-value


Gene annotations

Gene annotations based on NCBI database in the human and mouse genomes:

Genes in the human genome (NCBI build 36)

Genes in the mouse genome (NCBI build 37)

Each column displays:

-Start position

-End position

-Gene orientation

-Gene symbol


Figure 3

PDF format

TIFF format


Contact

If you have any question, please contact:

Zhongming Zhao

Email: zzhao@vcu.edu

Leng Han

Email: hanl2@vcu.edu

 

Last updated: November 25, 2008